The group has a long tradition for making biosensors based on DNA and proteins. The combination of DNA, redox-active molecules and conductive surfaces provides unique opportunities for making electrochemical sensors. Recently we have engaged in a new method to detect proteins and small molecules in solution by an optical readout.
Among our current sensor activities are:
Detection of small molecules and proteins in blood by the strand displacement reaction.
A DNA-Mediated Homogeneous Binding Assay for Proteins and Small Molecules. Zhang, Z.; Hejesen, C.; Kjelstrup, M. B.; Birkedal, V. B.; Gothelf, K. V. J. Am. Chem. Soc. 2014, 136, 11115-11120.
Some of our past contributions to this field are:
- Efficient colorimetric and fluorescent detection of fluoride in DMSO-water mixtures with arylaldoximes. Rosen, C. B.; Hansen, D. J.; Gothelf, K. V. Org. Biomol. Chem. 2013, 11, 7916-7922. DOI: 10.1039/c3ob41078a
- An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum. Ferapontova, E. E.; Olsen, E. M.; Gothelf, K. V. J. Am. Chem. Soc. 2008, 130, 4256–4258.
- An electrochemical sensor based on the human estrogen receptor ligand binding domain. Hansen, J. A.; Sumbayev, V. V.; Gothelf, K. V. Nano Lett. 2007, 7, 2831–2834.
- Femtomolar electrochemical detection of DNA targets using metal sulfide nanoparticles. Hansen, J. A.; Mukhopadhyay, R.; Hansen, J. Ø.; Gothelf, K. V. J. Am. Chem. Soc. 2006, 128, 3860–3861.