Templated Synthesis

DNA-templated synthesis provides the opportunity control chemical reactions with DNA-strands. The reactive groups are conjugated to DNA strands and the reactions performed at low concentrations. Only if the reactive groups are brought together by specific DNA-hybridization the local concentration becomes sufficiently high for the reaction to occur.

Current activities are

  • DNA Templated protein conjugation
  • Small molecule labeling of proteins and antibodies

Some of our past contributions to this field are

DNA template assembly of molecular wires:

  • DNA-programmed Glaser-Eglinton reactions for the synthesis of conjugated molecular wires. Ravnsbæk, J. B.; Jacobsen, M. F.; Rosen, C. B.; Voigt, N. V.; Gothelf, K. V. Angew. Chem. Int. Ed. 2011, 50, 10851–10854.
  • Bridging one helical turn in double-stranded DNA by templated dimerization of molecular rods. Andersen, C. S.; Yan, H.; Gothelf, K. V. Angew. Chem. Int. Ed. 2008, 47, 5569–5572.
  • Modular DNA-programmed assembly of linear and branched conjugated nanostructures. Gothelf, K. V.; Thomsen, A.; Nielsen, M.; Cló, E.; Brown, R. S. J. Am. Chem. Soc. 2004, 126, 1044–1046.

The yoctoreactor (In collaboration with Vipergen)

  • A yoctoliter-scale DNA reactor for small-molecule evolution. Hansen, M. H.; Blakskjær, P.; Petersen, L. K.; Hansen, T. H.; Højfeldt, J. W.; Gothelf, K. V.; Hansen, N. J. V. J. Am. Chem. Soc. 2009, 131, 1322–1327.
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