DNA Nanostructures

Unlike almost all other molecules the self-assembly of oligonucleotides can be programmed to fold into predesigned structures with high fidelity. In particular the development of the methods for DNA origami and DNA-tile assembly has allowed us to form highly complex self-assembled structures. Furthermore, by integrating strand-displacement reactions dynamic DNA structures can be formed.

Current projects in this area are:

  • Immobilization and physical properties of conjugated polymers in DNA structures.
  • New methods for immobilization of proteins in DNA nanostructures.
  • Formation of complex 3D structures for drug delivery.
  • Chemical stabilizing DNA nanostructures.

Some of our past contributions to this field are:

DNA origamiMethods for self-assembly of DNA nanostructures

  • Self-Assembly of DNA Origami and Single-Stranded Tile Structures at Room Temperature. Zhang, Z.; Song, J.; Besenbacher, F.; Dong, M.; Gothelf, K. V. Chem. Int. Ed. 2013, 52, 9219. DOI: 10.1002/anie.201303611
  • Isothermal Hybridization Kinetics of DNA Assembly of Two-Dimensional DNA Origami. Song, J.; Zhang, Z.; Zhang, S.; Liu, L.; Li, Q.; Xie, E. Q.; Gothelf, K. V.; Besenbacher, F.; Dong, M. Small. 2013, 9, 2954-2959. DOI: 1002/smll.201202861
  • Single-step rapid assembly of DNA origami nanostructures for addressable nanoscale bioreactors. Fu, Y.;, Zeng, D.; Chao, J.;, Zhang, Z.; Liu, H.; Ma, H.; Huang, Q.; Gothelf, K.; Fan, C. Am. Chem. Soc. 2013 135, 696-702.
  • Direct Visualization of Transient Thermal Response of a DNA Origami. Song, J.; Arbona, J. M. ; Zhang, Z.; Liu, L.; Xie, E.Q.; Elezgaray, J.; Aime, J. P.; Gothelf, K.V.; Besenbacher, F.; Dong, M. D. Am. Chem. Soc. 2012, 134, 9844-9847

A DNA Actuator

  • A DNA tile actuator with eleven discrete states. Zhang, Z.; Olsen, E. M.; Kryger, M.; Voigt, N. V.; Tørring, T.; Gültekin, E.; Nielsen, M.; MohammadZadegan, R.; Andersen, E. S.; Nielsen, M. M.; Kjems, J.; Birkedal, V.; Gothelf, K. V. Chem. Int. Ed. 2011, 50, 3983–3987.
  • Extended DNA Tile Actuators. Kristiansen, M.; Kryger, M. B. L.; Zhang, Z.; Voigt, N. V.; Birkedal,V. Gothelf, K. V. ChemPlusChem 2012, 77, 636–642.
  • Movement of a DNA Actuator Investigated by Single Molecule Fret Microscopy. Hildebrandt, L. L.; Zhang, Z.; Preus, S.; Gothelf, K. V.; Birkedal, V. Biophys. J. 2014, 106, 226A.
  • Single molecule FRET analysis of the 11 discrete steps of a DNA actuator. Hildebrandt, L. L.; Preus , S.; Zhang, Z.; Voigt, N. V.; Gothelf, K. V.; Birkedal, V. J. Am. Chem. Soc. 2014, 136, 8957–8962

Chemical reactionsChemical reactions on DNA origami

  • Single-molecule chemical reactions on DNA origami. Voigt, N. V.; Tørring, T.; Rotaru, A.; Jacobsen, M. F.; Ravnsbæk, J. B.; Subramani, R.; Mamdouh, W.; Kjems, J.; Mokhir, A.; Besenbacher, F.; Gothelf, K. V. Nanotech. 2010, 5, 200.
  • Single molecule atomic force microscopy studies of photosensitized singlet oxygen behavior on a DNA origami template. Helmig, S.; Rotaru, A.; Arian, D.; Kovbasyuk, L.; Arnbjerg, J.; Ogilby, P. R.; Kjems, J.; Mokhir, A.; Besenbacher, F.; Gothelf, K. V. ACS Nano 2010, 4, 7475–7480.
  • Singlet Oxygen in DNA Nanotechnology. Tørring, T.; Helmig, S.; Ogilby, P.; Gothelf, K. V. Chem. Res. 2014, 47, 1799–1806.

DNA boxDNA Box

  • Self-assembly of a nanoscale DNA box with a controllable lid. Andersen, E. S.; Dong, M.; Nielsen, M. M.; Jahn, K.; Subramani, R.; Mam- douh, W.; Golas, M. M.; Sander, B.; Stark, H.; Oliveira, C. L. P.; Pedersen, J. S.; Birkedal, V.; Besenbacher, F.; Gothelf, K. V.; Kjems, J. Nature 2009, 459, 73–76.
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