PAPER: DNA-templated covalent coupling of G4 PAMAM dendrimers

Liu, H.; Tørring, T.; Dong, M.; Rosen, C. B.; Besenbacher, F.; Gothelf, K. V.

J. Am. Chem. Soc. 2010, 132, 18054–18056, doi: 10.1021/ja109677n.

Centre for DNA Nanotechnology (CDNA) at The Interdisciplinary Nanoscience Center (iNANO), Aarhus University, DK-8000 Aarhus C, Denmark.


Generation-4 polyamidoamine (PAMAM) dendrimers were surface-functionalized with azides or alkynes and conjugated to one DNA strand. DNA-controlled self-assembly of alternating azide and alkyne dendrimers on a DNA template enabled the coupling of the dendrimers by the azide-alkyne “click” reaction to form covalently coupled dimers, trimers, and tetramers. Polymerization of the DNA-dendrimer conjugates was also demonstrated, as well as assembly in a circular structure on DNA origami and imaging by atomic force microscopy.

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