PAPER: Design and construction of double-decker tile as a route to three-dimensional periodic assembly of DNA

Majumder, U.; Rangnekar, A.; Gothelf, K. V.; Reif, J. H.; LaBean, T. H.

J. Am. Chem. Soc. 2011, 133, 3843–3845, doi: 10.1021/ja1108886

Departments of Computer Science, Chemistry, and Biomedical Engineering, Duke University, Durham, North Carolina 27708, United States

Centre for DNA Nanotechnology, Department of Chemistry and iNANO, Aarhus University, DK-8000 Århus, Denmark


DNA is a useful material for nanoscale construction. Due to highly specific Watson−Crick base pairing, the DNA sequences can be designed to form small tiles or origami. Adjacent helices in such nanostructures are connected via Holliday junction-like crossovers. DNA tiles can have sticky ends which can then be programmed to form large one-dimensional and two-dimensional periodic lattices. Recently, a three-dimensional DNA lattice has also been constructed. Here we report the design and construction of a novel DNA cross tile, called the double-decker tile. Its arms are symmetric and have four double helices each. Using its sticky ends, large two-dimensional square lattices have been constructed which are on the order of tens of micrometers. Furthermore, it is proposed that the sticky ends of the double-decker tile can be programmed to form a three-dimensional periodic lattice with large cavities that could be used as a scaffold for precise positioning of molecules in space.

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