PAPER: Self-Assembled Nanoparticles of Glycol Chitosan – Ergocalciferol Succinate conjugate, for controlled release

Quinones Pérez, J.; Gothelf , K. V.; Kjems, J.; Caballero, A. M. H.; Scmidt, C.; Covas, C. P.

Carbohyd. Polym. 2012, 88, 1373-1377. DOI: 10.1016/j.carbpol.2012.02.039

  • Center of Natural Products, Faculty of Chemistry, University of Havana, Havana, Cuba
  • Interdisciplinary Nanoscience Center iNANO/Institut for Kemi, University of Aarhus, Aarhus, Denmark
  • Interdisciplinary Nanoscience Center iNANO/Department of Molecular Biology, University of Aarhus, Aarhus, Denmark
  • Instituto de Estudios Biofuncionales/Dpto Química Física II, Facultad de Farmacia, Universidad Complutense de Madrid, Spain
  • Department of Chemistry, University of Paderborn, Paderborn, Germany
  • Center of Biomaterials, University of Havana, Havana, Cuba


Glycol chitosan was linked to vitamin D2 hemisuccinate (ergocalciferol hemisuccinate) for controlled release through water-soluble carbodiimide activation. The resulting conjugate formed self-assembled nanoparticles in aqueous solution with particle size of 279 nm and ergocalciferol hemisuccinate content of 8.4% (w/w). Almost spherical 50–90 nm nanoparticles were observed by scanning and transmission electron microscopy upon drying. Drug linking to glycol chitosan was confirmed by FTIR spectroscopy and proton NMR. Particles were also characterized by differential scanning calorimetry and wide-angle X-ray diffraction studies. In vitro vitamin D2 release studies performed in water at acid pH indicated a drug release dependence on the solution acidity. Almost constant release rate was observed during the first 8 h. These results indicate that the obtained nanoparticles could be good candidates for vitamin D2 release to animals and humans.

%d bloggers like this: