Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate

Busuttil K, Rotaru A, Dong M, Besenbacher F, Gothelf KV.

Chem Commun (Camb). 2013 Mar 7;49(19):1927-9. doi: 10.1039/c3cc37408d. Epub 2013 Jan 29.

Danish National Research Foundation: Centre for DNA Nanotechnology at Interdisciplinary Nanoscience Center, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark.


Patterns of the protein streptavidin on DNA origami are printed onto a functionalized surface. The streptavidins are linked to DNA via a disulfide spacer and these conjugates self-organise into a predetermined pattern on DNA origami. The structure immobilises on a biotin-SAM-functionalized Au surface. By disulfide cleavage the origami is separated from the streptavidins, leaving the protein pattern at the surface.

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